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enzyme linked immunosorbent elisa assay  (R&D Systems)


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    R&D Systems enzyme linked immunosorbent elisa assay
    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by <t>ELISA.</t> ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
    Enzyme Linked Immunosorbent Elisa Assay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunosorbent+assay+elisa+plate+sealers/ELISA+Plate+Sealers/pmc13046777-341-12-24
    Average 94 stars, based on 27 article reviews
    enzyme linked immunosorbent elisa assay - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discovery"

    Article Title: Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discovery

    Journal: Scientific Reports

    doi: 10.1038/s41598-026-40196-0

    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by ELISA. ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
    Figure Legend Snippet: Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by ELISA. ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).

    Techniques Used: Formulation, Enzyme-linked Immunosorbent Assay, Zeta Potential Analyzer, Standard Deviation

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    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by <t>ELISA.</t> ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
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    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by <t>ELISA.</t> ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
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    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by <t>ELISA.</t> ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
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    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by <t>ELISA.</t> ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
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    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by <t>ELISA.</t> ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
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    Image Search Results


    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by ELISA. ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).

    Journal: Scientific Reports

    Article Title: Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discovery

    doi: 10.1038/s41598-026-40196-0

    Figure Lengend Snippet: Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by ELISA. ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).

    Article Snippet: The exact concentrations of the solutions were determined using a commercially available enzyme-linked immunosorbent (ELISA) assay (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems).

    Techniques: Formulation, Enzyme-linked Immunosorbent Assay, Zeta Potential Analyzer, Standard Deviation